Affinity separation polymer of lactoferrin and affinity purification method of lactoferrin

The invention relates to a compatible separating material of lactoferrin in the technical field of biomedicine and a compatible purification method of lactoferrin. The method includes the following steps: step one, the compatible separating material dedicated for lactoferrin is synthesized; firstly,...

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Bibliographische Detailangaben
Hauptverfasser: HUANG FEIYUN, REN HUIJUAN, HUO CHENXI, WEN SHENG, LI RONGXIU
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a compatible separating material of lactoferrin in the technical field of biomedicine and a compatible purification method of lactoferrin. The method includes the following steps: step one, the compatible separating material dedicated for lactoferrin is synthesized; firstly, a basic chromatography medium with amido or a conventional chemical method is used for derivatization to the basic chromatography medium, and the amido is carried for activating reaction with trichloro-triazine; and then the basic chromatography medium reacts with beta-lactamine or 1-aminocyclohexane methanoic acid to synthesize the compatible separating material; step two, biomimetic compatible separating material prepared is used for purifying the lactoferrin. The material and the method canlead to mass production with high-purity lactoferrin, high efficiency and low cost.