RNA interference vector and use thereof

The present invention provides a RNA interference vector and an application thereof on genetically modified nematode-resistant plants, which belong to the technical field of plant protection. The RNA interference vector is characterized in that a plant expression vector contains a nucleotide sequenc...

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Bibliographische Detailangaben
Hauptverfasser: FENG DONGXIN, YANG YUHONG, XIE BINGYAN, FENG LANXIANG, CHEN GUOHUA, MAO ZHENCHUAN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The present invention provides a RNA interference vector and an application thereof on genetically modified nematode-resistant plants, which belong to the technical field of plant protection. The RNA interference vector is characterized in that a plant expression vector contains a nucleotide sequence which is more than 74 percent similar to a sequence shown by Sequence No:1 and has the same function as the protein expressed by the Sequence No:1 sequence does, and the nucleotide sequence is inserted into the plant expression vector in the positive and negative directions. The constructed vector transforms a receptor plant; the transcribed exogenous gene can form a foldback double-stranded hairpin loop RNA structure; after the genetically modified plant is eaten by nematode, the target gene in the body of the nematode, MiMPK1 gene, interacts with the dsRNA expressed by the exogenous gene in the body of the plant, so that a silencing effect occurs; and as a result, the nematode cannot normally develop and breed.