Fast detecting method for total number of bacterial colony in sauce

The invention discloses a rapid detecting method for detecting the total number of colonies in soy sauce. The method comprises the following steps: firstly, enriched thalli are separated by using the membrane separation technique, and substances affecting the growth and a luminescence system of the...

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Bibliographische Detailangaben
Hauptverfasser:	PAN SIYI, HUANG WENBIAO, YAN SHOULEI, ZOU MINJUAN, MIAO SUNA
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a rapid detecting method for detecting the total number of colonies in soy sauce. The method comprises the following steps: firstly, enriched thalli are separated by using the membrane separation technique, and substances affecting the growth and a luminescence system of the thalli are removed; secondly, a culture medium is used for repairing sublethal thalli and changing the spore into trophozoite cells, so the thalli are in sync growth status; and thirdly, a bacterial suspension and an ATP luminescence agent are reacted, so the total number of the colonies can be detected according to the light value. The invention discloses the culture medium used for detecting the total number of the colonies in soy sauce quickly. The detecting method is rapid, the total number of colonies can be detected accurately within 4 hours, and the invention has the advantages of promptness and accuracy.