Non-virus non-structural protein foot-and-mouth disease vaccine and preparation thereof

The invention discloses a method for preparing a foot and mouth disease vaccine. The preparation method comprises the steps that monoclonal antibodies of non-structure protein of foot and mouth disease virus is used for removing non-structure protein of foot and mouth disease virus in a target object which is then prepared into the foot and mouth disease vaccine, wherein, the target object is a foot and mouth disease virus culture or a gene engineering expression product comprising the non-structure protein of foot and mouth disease virus and structure protein of foot and mouth disease virus.The reliability is greatly improved when infected animals are distinguished from immune animals in vaccine immunization animals using the foot and mouth disease vaccine of the invention; in addition,the method for preparing the foot and mouth disease vaccine of virus-free non-structure protein is simple, convenient and effective without extra expensive equipment and can be applied to applicationon a large scale. Therefore