Method for preparing biodiesel with immobile surfactant-coated enzyme

Disclosed is a method of producing bio-diesel based on immobile surfactant-coated enzymes; under the effect of the catalyst immobile surfactant-coated enzymes and certain temperature, lower alcohol and animal and vegetable oils are mixed; and then the organic solvent normal hexane and water are added; the mixture is stirred for the ester interchange reaction; the upper layer is the crude product of bio-diesel and the lower layer is mainly the glycerol after the reaction; the crude product of bio-diesel in the upper layer is purified to obtain the bio-diesel; the method of producing bio-diesel based on immobile surfactant-coated enzymes has the advantages of adopting immobile surfactant-coated enzymes for the production of the bio-diesel, effective and mild reaction conditions.