Fused protein of human serum albumin and human insulin C-peptide, and preparation thereof

The invention relates to a preparation method of a fused protein of human serum albumin and human insulin C peptide, and the product thereof, belonging to the technical field of long-effetive recombinant fused protein drugs. Human serum albumin(HSA) cDNA and C peptide cDNA are directly connected, without adding any connecting peptide therevetween, obtaining HAS-CP cDNA which is then integrated with a cell of the host to express. The fused protein of the invention comprises a first region with at least 85% of the sequence congenetic with human serum albumin and a second region with at least 85% of the sequence congenetic with human C peptide, and the two regions are directly connected without any connecting peptide therebetween. Under the premise of not being changed in characteristics, the fused protein is capable of the substitution, deletion or addition of specific amino acid residues. The host cell can be bacteria, barm, insect cell, zooblast, plant cell, etc. The fused protein maintains the physiological