Rapeseed cultivar stem segment tissue culture fast breeding male sterile lines seed making method

The invention provides a tissue culture and rapid propagation nuclear male sterile line seed preparation method utilizing the stem of rapeseed, which aims to solve the drawbacks in prior art of low transplanting survival rate of tissue culture seedlings, bad encounter of the tissue culture seedlings and the florescence of seed plants and no corresponding seed preparation method. Compound fertilizer and farm manure are selected as the field base fertilizer; the row ratio of male parent and tissue culture seedlings is 1: 3 to 4; after 0 days to five days of the transplantation of the tissue culture seedlings, male parent seeds are sown, and the tissue culture seedlings are directly transplanted to the field; the tissue culture seedlings cannot contact the base fertilizer; root fixation water is applied in no time after transplantation; the tissue culture seedlings are covered by a thin film for moisture retention for 4 days to 7 days; 10 to 15 days after transplantation, urea dissolved in clean liquid manure is