Kandelia candel mangrove metallothionein gene and coded sequence thereof
The invention provides a bruguiera gymnorrhiza metallothionein and an encoded sequence thereof, cDNA sequence encoding protein contains base pairs from the 89th position to the 328th position of SED ID NO.1, which belongs to new plant type II metallothionein. The invention also relates to a method f...
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| creator | SUN CUICI WANG YOUSHAO ZHANG FENGQIN |
| description | The invention provides a bruguiera gymnorrhiza metallothionein and an encoded sequence thereof, cDNA sequence encoding protein contains base pairs from the 89th position to the 328th position of SED ID NO.1, which belongs to new plant type II metallothionein. The invention also relates to a method for separating nucleotide and the expression of bMT protein in escherichia coli. A DNA sequence which encodes the bMT is recombined to a plasmid vector pET-100TOPO and transformed into escherichia coli BL21, and the resistance is researched in LB culture medium which respectively contains heavy metal with different concentration. Strains which have the bMT can resist Zn2+and Cd2+ with certain concentration. The research results of the metallothionein relate to the fields such as agriculture, medicine, biochemistry, molecular biology, environmental science, hygienic toxicology, food science, nutriology, health science, methodology and the like, and the research results not only can develop various therapeutic drugs, |
| format | Patent |
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The invention also relates to a method for separating nucleotide and the expression of bMT protein in escherichia coli. A DNA sequence which encodes the bMT is recombined to a plasmid vector pET-100TOPO and transformed into escherichia coli BL21, and the resistance is researched in LB culture medium which respectively contains heavy metal with different concentration. Strains which have the bMT can resist Zn2+and Cd2+ with certain concentration. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Kandelia candel mangrove metallothionein gene and coded sequence thereof |
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