Primer and probe of west nile virus and real time RT-PCR detection reagent kit with one-step method

The invention relates to a group of primers and probes and a corresponding kit, including a pair of primers which can simultaneously detect West Nile virus and the reference gene and two TaqMan probes against the West Nile virus and the reference gene, so as to reduce the number of the primers and the probes in a PCR reaction system from the usual 6 to 4, further to realize the high efficient amplification of the West Nile virus and the reference gene in the same tube, thus realizing the detection of the target genes of the different species, having strong universality, reducing the number of the primers and the probes, the cost of the reagents and the operation steps, and reducing the pollution chances which need to be avoided generally in a PCR experiment. The kit of the invention is applicable to the detection of the West Nile virus in the scientific research or the clinical aspect, especially the monitoring of the West Nile virus in the prevention and the control works of viral diseases.