Method for identification of Szechwan Ligusticum wallichii
The invention provides an identification method of Sichuan chuanxiong rhizome, which comprises using the aqueous extractor of chuanxiong rhizome as sample solution, processing HPCE analysis, while the electrophoresis conditions comprise the capillary is eCAPTM(75umx60cm, the effective length is 50cm...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention provides an identification method of Sichuan chuanxiong rhizome, which comprises using the aqueous extractor of chuanxiong rhizome as sample solution, processing HPCE analysis, while the electrophoresis conditions comprise the capillary is eCAPTM(75umx60cm, the effective length is 50cm), the buffer solution is 30mmol/L borax containing 5% acetonitrile, using NaOH to adjust pH to 9.60, feeding sample under pressure at 137.9kpa for 5sec, while the separation conditions are 25KV and 20DEG C, the checking wavelength is 295nm, obtaining chuanxiong rhizome HPCE fingerprint pattern provided with a characteristic peak presented in the table 1, judging the object chuanxiong rhizome as Sichuan chuanxiong rhizome. The invention has the advantages of simple, stable and easy sample preparation, short analysis time, low organic solvent consumption, low cost and environment protection, which can effectively identify Sichuan chuanxiong rhizome via fingerprint pattern, to confirm stable,uniform and controllable |
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