Liquid phase chip reagent box for early diagnosing mammary cancer and preparation method thereof

The invention discloses a liquid phase chip kit used for early diagnosis of breast cancer, which primarily comprises: (1) 5-plex coated microsphere comprising a No.51 microsphere coated with an IL-6 capture antibody, a No. 38 microsphere coated with an IL-8 capture antibody, a No. 34 microsphere coated with a TNF-Alpha capture antibody, a No. 20 microsphere coated with a T-PSA capture antibody, a No. 32 microsphere coated with a VEGF capture antibody; (2) intermixture to detect antibodies, which is marked with biomarkers of IL-6, IL-8; TNF-Alpha, T-PSA and VEGF; (3) streptavidin phycoerythrin. The invention discloses a method to prepare the liquid phase chip kit. The liquid phase chip kit used for early diagnosis of breast cancer has the advantages of no side effect, high flux, multiple indices used for parallel detection, high sensitivity, high repetitiveness, quick and accurate detection, low cost and so on.