Rapid separation and purification method of cordyceps militaris fruit body water-soluble peptide polysaccharide
The invention discloses a rapid separation and purification method of the water soluble peptide polysaccharide from Cordyceps Militaris and belongs to the extraction technology field of the bioactive substances. The method comprises drying and smashing the Cordyceps Militaris, obtaining the componen...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a rapid separation and purification method of the water soluble peptide polysaccharide from Cordyceps Militaris and belongs to the extraction technology field of the bioactive substances. The method comprises drying and smashing the Cordyceps Militaris, obtaining the component CF1 by extracting the ultrapure water, obtaining the component CF1-1 by the separation from the molecular sieve, obtaining the component CF1-1-1 and CF1-1-2 by separation and purification from the ionic exchange column, and obtaining the CF1-1-1-1, CF1-1-2-1 and CF1-1-2-2 by separation from the affinity chromatography column. By adopting the purification method of smashing the Cordyceps Militaris and direct introduction of the water extract on column, the invention greatly promotes the efficiency and decreases the cost, in addition, the entire separation and purification process does not need the organic solvent, thereby really achieving the purpose of green, rapidness and high efficiencyof the separation of the |
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