Gathering and processing method of cone needed by cunninghamia lanceolata somatic embryo inducement
The invention discloses a collecting and treating method of cone required for fir somatic embryo induction. Immature cones are collected 107-149 days after a fir is hybridized and pollinated; the cones collected are wrapped with wet cloth, sealed and put into a refrigerator with a controlled tempera...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a collecting and treating method of cone required for fir somatic embryo induction. Immature cones are collected 107-149 days after a fir is hybridized and pollinated; the cones collected are wrapped with wet cloth, sealed and put into a refrigerator with a controlled temperature of 4-8 DEG C for refrigerating for 0-50 days; the refrigerated cones are taken out, and seeds are taken out of the cones and sterilized; the sterilized seeds are dried and peeled, and the obtained seeds are suitable for being inoculated onto a culture medium that is inducted by fir somatic embryo for forming ESM. Therefore, high inductivity is acquired, and the method can be combined with other technologies, and can fully meet the requirement of industrialization production of the fir somatic embryo, thereby realizing the industrialization production of the fir. |
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