Potato detoxicatubg planting potato asepsis circulating germplasm preserving method
The present invention relates to a potato detoxified sprout sterile circulation germ plasm preservation method. Said method includes the following steps: using existent tuber tip tissue culture technique to obtain detoxified sprout, utilizing induction process to obtain test tube miniature potato, a...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The present invention relates to a potato detoxified sprout sterile circulation germ plasm preservation method. Said method includes the following steps: using existent tuber tip tissue culture technique to obtain detoxified sprout, utilizing induction process to obtain test tube miniature potato, and it is characterized by that placing said test tube miniature potato into a culture vessel, making low-temperature preservation for 200-360 days at 3-5deg.C, then taking out said test tube miniature potato, under the condition of that the temperature is 20-25deg.C and illumination is 2000-4000 lux making culture for 40-60 days to obtain sterile sprout, under the condition of that temperature is 18-20deg.C culturing said sterile sprout for 60-80 days to obtain miniature potato, repeating above-mentioned operation so as to implement the detoxified potato sprout circulation germ plasm preservation. |
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