Tissue cultivation quick breeding method of cyclamen

The invention discloses a method of tissue culture and rapid propagation for cyclamen persicum, which is divided into the following steps: (1) leaf of flowered cyclamen persicum is taken as explants to be inoculated to induction culture medium to induce callus; (2) the callus is transplanted to diff...

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Bibliographische Detailangaben
Hauptverfasser: QU FUNING, YOU CUIRONG, GONG XUEQIN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a method of tissue culture and rapid propagation for cyclamen persicum, which is divided into the following steps: (1) leaf of flowered cyclamen persicum is taken as explants to be inoculated to induction culture medium to induce callus; (2) the callus is transplanted to differentiation culture medium to cultivate adventitious shoot and then the adventitious shoot is selected as multiplication organ and after successive generations rootless tube plant is obtained; (3) the rootless tube plant is transferred to rooting culture medium to cultivate rooted tube plant; (4) the plant after being strengthened is transplanted in flowerpot or garden and then is managed according to routine method. Compared with seedling plant, the tissue cultivated plant of the invention has more leaves, good leaf coverage, compact plant, more flowering, and formal and better appearance. In addition, the method of the invention can recover and maintain the species property of cyclamen persicum, ensure formality