Method for diagnosing sporangium and egg capsule molecules of sarcocyst

This invention relates to a method for detecting sporangium and oocyst of Sarcocystis. The method comprises: (1) mixing a small amount of feces with 37% ZnSO4 solution, centrifuging and standing; (2) detecting floating feces with a microscope, collecting sporangium and oocyst, placing in centrifugal...

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Bibliographische Detailangaben
Hauptverfasser:	LEI LIN, YANG ZHAOQING, XIANG ZHENG, CHEN XINWEN, ZHANG YAPING, ZUO YANGXIAN, ZHOU BENJIANG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	This invention relates to a method for detecting sporangium and oocyst of Sarcocystis. The method comprises: (1) mixing a small amount of feces with 37% ZnSO4 solution, centrifuging and standing; (2) detecting floating feces with a microscope, collecting sporangium and oocyst, placing in centrifugal tube, and storing at a low temperature; (3) performing PCR with the primers to amplify 18SrRNA in sporangium and oocyst of Sarcocystis; (4) digesting PCR product with restriction endonuclease, and detecting through gel electrophoresis, or detecting the nucleotide sequence with the primers used before. The primers can also be used as probes in hybridization reactions, or for manufacturing gene chips or microarrays. The method can be used for identifying Sarcocystis with high sensitivity and high specificity.