Tissue cultivating and seedling method of fast growing anthocephalus

The present invention discloses a tissue culture process for raising seedling of fast growing Anthocephalus chinensis. The tissue culture process includes the following steps: taking bud stick as explant, sterilizing and cleaning; inducing culturing in inducing culture medium comprising 1/2MS, 6-BA 0.8-1.0 mg/L and NAA 0.2-0.3 mg/L at illumination intensity of 1000+/-500 Lux; secondary culturing in secondary culture medium comprising MS, 6-BA 0.3-0.5 mg/L and NAA 0.1-0.2 mg/L at illumination intensity of 2500-3000 Lux; rooting culturing in rooting culture medium comprising MS and NAA 0.1-0.3 mg/L at illumination intensity of 2500-3500 Lux for about 25 days to obtain bottle seedling of 3-4 cm height and 0.5-1.0 cm root length; and transplanting to mixed medium comprising vermiculite and turfin the volume ratio of 2 to 3. The process has culture period of 120 days, survival rate over 96 % and low production cost, and is suitable for large scale production.