PCR inspection of tubercle bacillus in dairy product

A method for testing tuberculine composite bacterial population in dairy by real-time fluorescent PCR is carried out by adding BCG into emulsion as analog clinical testing samples, extracting DNA, real-time fluorescent PCR inspecting based on TaqMan technology, selecting ITS sequence as cloning sequ...

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1. Verfasser:	MINGTAO,DAI DU
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method for testing tuberculine composite bacterial population in dairy by real-time fluorescent PCR is carried out by adding BCG into emulsion as analog clinical testing samples, extracting DNA, real-time fluorescent PCR inspecting based on TaqMan technology, selecting ITS sequence as cloning sequence target sequence between tuberculine with code 16sRNA gene and 23sRNA gene. It's safe and has better sensitivity. It can be used to prevent human and domestic animal and diagnose cow tuberculosis.