Metodo para producir, aislar y purificar antitriptasa humana recombinante (osraat) a partir de semillas de arroz

GEN OsrAAT (ANTITRIPSINA ALFA 1 HUMANA OPTIMIZADA PARA Oryza sativa) DE CODÓN GENÉTICO OPTIMIZADO DE ARROZ; VECTOR QUE LO COMPRENDE; USO PARA TRANFORMAR SEMILLAS DE ARROZ; MÉTODO PARA PREPARAR SEMILLAS DE ARROZ TRANSGÉNICO; Y MÉTODO PARA AISLAR Y PURIFICAR OsrATT. Provided in the present invention a...

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Hauptverfasser:	SHI QIANNI, YANG DAICHANG, ZHANG LIPING
Format:	Patent
Sprache:	spa
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM ORGANIC CHEMISTRY PEPTIDES PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	GEN OsrAAT (ANTITRIPSINA ALFA 1 HUMANA OPTIMIZADA PARA Oryza sativa) DE CODÓN GENÉTICO OPTIMIZADO DE ARROZ; VECTOR QUE LO COMPRENDE; USO PARA TRANFORMAR SEMILLAS DE ARROZ; MÉTODO PARA PREPARAR SEMILLAS DE ARROZ TRANSGÉNICO; Y MÉTODO PARA AISLAR Y PURIFICAR OsrATT. Provided in the present invention are a rice genetic codon-optimized OsrAAT gene, related vector and a method for expression of OsrAAT in transgenic rice seeds by using the vector and a method for isolating and purifying OsrAAT. A transgenic rice seed expressing OsrAAT is prepared by the endosperm-specific expression vector. The purity of OsrAAT isolated and purified from transgenic rice grains reached 97% with HPLC grade and the yield of OsrAAT was up to 18.89 ± 3.19% of total soluble proteins, thus corresponding that 0.366 g of OsrAAT per kilogram can be produced from brown rice.