Immobilisierte Enzyme
Immobilised enzymes are adsorptively immobilised on enzyme carriers consisting of particles of unmodified natural substance, mainly of a cellulose nature. Pref. the carrier is sawdust, cellulose fibres or cotton lint. The particlesare 0.01-1mm. Enzyme is a hydrolase, isomerase, oxido-reductase or tr...
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Zusammenfassung: | Immobilised enzymes are adsorptively immobilised on enzyme carriers consisting of particles of unmodified natural substance, mainly of a cellulose nature. Pref. the carrier is sawdust, cellulose fibres or cotton lint. The particlesare 0.01-1mm. Enzyme is a hydrolase, isomerase, oxido-reductase or transferase, esp. a lipase (E.C.3.1.1.3), protease (E.C.3.4) or catalase (E.C.1.11.1.6). The immobilised enzymes can be prepd. by allowing the enzyme to be drawn up into the unmodified natural substance particles from aq. medium. The aq. medium contains 1/5 to 1/15 of its wt. of regulating salt, esp. sodium sulphate. USE/ADVANTAGE - The fibres formed from the natural substances are not chemically reactive and need no activation. Immobilised enzymes can be used in organic media for enzyme catalysed reactions and retain their full activity. Immobilised enzymes are esp. suitable for use in enantioselective reesterifications |
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