PROCESS FOR PRODUCING STEROID DEHYDROGENASE

1291183 Steroid dehydrogenase KYOWA HAKKO KOGYO KK 6 May 1970 [6 May 1969] 21909/70 Heading C3H [Also in Division C2] Steroid dehydrogenase is produced by culture of a microorganism of the species Brevibacterium sterolicum in a nutrient medium, and recovering the enzyme from the bacterial cells and...

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Bibliographische Detailangaben
Hauptverfasser:	HITOSHI YAGI, OSAMU TERADA, SHIZUKO NAKAMURA
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Zusammenfassung:	1291183 Steroid dehydrogenase KYOWA HAKKO KOGYO KK 6 May 1970 [6 May 1969] 21909/70 Heading C3H [Also in Division C2] Steroid dehydrogenase is produced by culture of a microorganism of the species Brevibacterium sterolicum in a nutrient medium, and recovering the enzyme from the bacterial cells and or culture medium. The microorganism may be Brevibacterium sterolicum ATCC 21387. The nutrient medium may contain cholesterol. The enzyme can dehydrogenate the 3#-hydroxy group in a steroid (and also the 17#-hydroxy group, if such is present) e.g. cholesterol may be converted to cholesterone. The enzyme may be removed from the cell-free culture medium by salting out (e.g. with ammonium sulphate) or precipitation (e.g. with acetone), or may be extracted from the bacterial cells using ethyl acetate.