COMPOSITIONS AND METHODS FOR PRODUCING REBAUDIOSIDE D
The present disclosure provides enzymes and a method to use those enzymes to transfer a sugar moiety to a substrate steviol glycoside. Specifically, designed beta-1,2-glycosyltransferases and sucrose synthases are used in a one-pot reaction to convert stevioside and Reb A into Reb E and Reb D. La pr...
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| creator | NANCE, MARK BAN, YIH-EN ANDREW KRISTIANSEN, NIKLAS DALGAS HAVRANEK, JAMES J DEVKOTA, ASHWINI ZANGHELLINI, ALEXANDRE GRABS, DANIELA ROBERTS, KYLE EUGENE |
| description | The present disclosure provides enzymes and a method to use those enzymes to transfer a sugar moiety to a substrate steviol glycoside. Specifically, designed beta-1,2-glycosyltransferases and sucrose synthases are used in a one-pot reaction to convert stevioside and Reb A into Reb E and Reb D.
La présente invention concerne des enzymes et un procédé d'utilisation de ces enzymes pour transférer une fraction de sucre à un glycoside de stéviol substrat. Spécifiquement, les bêta-1,2-glycosyltransférases et les saccharose synthases modifiées sont utilisées dans une réaction monotope pour convertir le stévioside et le Reb A en Reb E et Reb D. |
| format | Patent |
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La présente invention concerne des enzymes et un procédé d'utilisation de ces enzymes pour transférer une fraction de sucre à un glycoside de stéviol substrat. Spécifiquement, les bêta-1,2-glycosyltransférases et les saccharose synthases modifiées sont utilisées dans une réaction monotope pour convertir le stévioside et le Reb A en Reb E et Reb D.</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220825&DB=EPODOC&CC=CA&NR=3208720A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220825&DB=EPODOC&CC=CA&NR=3208720A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>NANCE, MARK</creatorcontrib><creatorcontrib>BAN, YIH-EN ANDREW</creatorcontrib><creatorcontrib>KRISTIANSEN, NIKLAS DALGAS</creatorcontrib><creatorcontrib>HAVRANEK, JAMES J</creatorcontrib><creatorcontrib>DEVKOTA, ASHWINI</creatorcontrib><creatorcontrib>ZANGHELLINI, ALEXANDRE</creatorcontrib><creatorcontrib>GRABS, DANIELA</creatorcontrib><creatorcontrib>ROBERTS, KYLE EUGENE</creatorcontrib><title>COMPOSITIONS AND METHODS FOR PRODUCING REBAUDIOSIDE D</title><description>The present disclosure provides enzymes and a method to use those enzymes to transfer a sugar moiety to a substrate steviol glycoside. Specifically, designed beta-1,2-glycosyltransferases and sucrose synthases are used in a one-pot reaction to convert stevioside and Reb A into Reb E and Reb D.
La présente invention concerne des enzymes et un procédé d'utilisation de ces enzymes pour transférer une fraction de sucre à un glycoside de stéviol substrat. Spécifiquement, les bêta-1,2-glycosyltransférases et les saccharose synthases modifiées sont utilisées dans une réaction monotope pour convertir le stévioside et le Reb A en Reb E et Reb D.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDB19vcN8A_2DPH09wtWcPRzUfB1DfHwdwlWcPMPUggI8ncJdfb0c1cIcnVyDHXxBKp0cVVw4WFgTUvMKU7lhdLcDApuriHOHrqpBfnxqcUFicmpeakl8c6OxkYGFuZGBo6GxkQoAQCpRCci</recordid><startdate>20220825</startdate><enddate>20220825</enddate><creator>NANCE, MARK</creator><creator>BAN, YIH-EN ANDREW</creator><creator>KRISTIANSEN, NIKLAS DALGAS</creator><creator>HAVRANEK, JAMES J</creator><creator>DEVKOTA, ASHWINI</creator><creator>ZANGHELLINI, ALEXANDRE</creator><creator>GRABS, DANIELA</creator><creator>ROBERTS, KYLE EUGENE</creator><scope>EVB</scope></search><sort><creationdate>20220825</creationdate><title>COMPOSITIONS AND METHODS FOR PRODUCING REBAUDIOSIDE D</title><author>NANCE, MARK ; BAN, YIH-EN ANDREW ; KRISTIANSEN, NIKLAS DALGAS ; HAVRANEK, JAMES J ; DEVKOTA, ASHWINI ; ZANGHELLINI, ALEXANDRE ; GRABS, DANIELA ; ROBERTS, KYLE EUGENE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CA3208720A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2022</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>NANCE, MARK</creatorcontrib><creatorcontrib>BAN, YIH-EN ANDREW</creatorcontrib><creatorcontrib>KRISTIANSEN, NIKLAS DALGAS</creatorcontrib><creatorcontrib>HAVRANEK, JAMES J</creatorcontrib><creatorcontrib>DEVKOTA, ASHWINI</creatorcontrib><creatorcontrib>ZANGHELLINI, ALEXANDRE</creatorcontrib><creatorcontrib>GRABS, DANIELA</creatorcontrib><creatorcontrib>ROBERTS, KYLE EUGENE</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>NANCE, MARK</au><au>BAN, YIH-EN ANDREW</au><au>KRISTIANSEN, NIKLAS DALGAS</au><au>HAVRANEK, JAMES J</au><au>DEVKOTA, ASHWINI</au><au>ZANGHELLINI, ALEXANDRE</au><au>GRABS, DANIELA</au><au>ROBERTS, KYLE EUGENE</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>COMPOSITIONS AND METHODS FOR PRODUCING REBAUDIOSIDE D</title><date>2022-08-25</date><risdate>2022</risdate><abstract>The present disclosure provides enzymes and a method to use those enzymes to transfer a sugar moiety to a substrate steviol glycoside. Specifically, designed beta-1,2-glycosyltransferases and sucrose synthases are used in a one-pot reaction to convert stevioside and Reb A into Reb E and Reb D.
La présente invention concerne des enzymes et un procédé d'utilisation de ces enzymes pour transférer une fraction de sucre à un glycoside de stéviol substrat. Spécifiquement, les bêta-1,2-glycosyltransférases et les saccharose synthases modifiées sont utilisées dans une réaction monotope pour convertir le stévioside et le Reb A en Reb E et Reb D.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING SPIRITS VINEGAR WINE |
| title | COMPOSITIONS AND METHODS FOR PRODUCING REBAUDIOSIDE D |
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