COMPOSITIONS AND METHODS FOR PRODUCING REBAUDIOSIDE D

COMPOSITIONS AND METHODS FOR PRODUCING REBAUDIOSIDE D

The present disclosure provides enzymes and a method to use those enzymes to transfer a sugar moiety to a substrate steviol glycoside. Specifically, designed beta-1,2-glycosyltransferases and sucrose synthases are used in a one-pot reaction to convert stevioside and Reb A into Reb E and Reb D. La pr...

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Bibliographische Detailangaben
Hauptverfasser: NANCE, MARK, BAN, YIH-EN ANDREW, KRISTIANSEN, NIKLAS DALGAS, HAVRANEK, JAMES J, DEVKOTA, ASHWINI, ZANGHELLINI, ALEXANDRE, GRABS, DANIELA, ROBERTS, KYLE EUGENE
Format: Patent
Sprache:eng ; fre
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The present disclosure provides enzymes and a method to use those enzymes to transfer a sugar moiety to a substrate steviol glycoside. Specifically, designed beta-1,2-glycosyltransferases and sucrose synthases are used in a one-pot reaction to convert stevioside and Reb A into Reb E and Reb D. La présente invention concerne des enzymes et un procédé d'utilisation de ces enzymes pour transférer une fraction de sucre à un glycoside de stéviol substrat. Spécifiquement, les bêta-1,2-glycosyltransférases et les saccharose synthases modifiées sont utilisées dans une réaction monotope pour convertir le stévioside et le Reb A en Reb E et Reb D.