METHODS OF OLIGONUCLEOTIDE-BASED AFFINITY CHROMATOGRAPHY

Disclosed are methods for purifying polynucleotides from a feed solution using oligonucleotide affinity columns at flowrates between 0.5 CV/min to 1000 CV/min. The methods can include several steps including loading a mixture containing the target polynucleotide onto a chromatography media, e.g., a...

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Bibliographische Detailangaben
Hauptverfasser:	ZHOU, JINXIANG, TEMPLES, GRAHAM
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES DERIVATIVES THEREOF ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PEPTIDES PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS SUGARS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Disclosed are methods for purifying polynucleotides from a feed solution using oligonucleotide affinity columns at flowrates between 0.5 CV/min to 1000 CV/min. The methods can include several steps including loading a mixture containing the target polynucleotide onto a chromatography media, e.g., a column, that carries a macroporous support with an oligonucleotide affinity ligand bonded to a surface. The affinity ligand can hybridize the targeted polynucleotide and allow for separation and purification of the target. L'invention divulgue des procédés de purification de polynucléotides à partir d'une solution de charge au moyen de colonnes d'affinité d'oligonucléotides à des débits entre 0,5 VC/min et 1 000 VC/min. Les procédés peuvent comprendre plusieurs étapes, comprenant le chargement d'un mélange contenant le polynucléotide cible sur un milieu chromatographique, par exemple une colonne, qui porte un support macroporeux à une surface duquel est lié un ligand d'affinité d'oligonucléotide. Le ligand d'affinité peut hybrider le polynucléotide ciblé et permettre la séparation et la purification de la cible.