PURIFICATION OF SULFONATED DNA

The present disclosure provides methods and systems for purification of chemically modified, specifically, sulfonated DNA. Additionally, the disclosure provides methods and systems for bisulfite conversion with improved purification of sulfonated DNA using silica supports, such as silica beads, and...

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Bibliographische Detailangaben
Hauptverfasser:	GAGRAT, ZUBIN, DOMANICO, MICHAEL J, RAGLAND, EVAN M
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present disclosure provides methods and systems for purification of chemically modified, specifically, sulfonated DNA. Additionally, the disclosure provides methods and systems for bisulfite conversion with improved purification of sulfonated DNA using silica supports, such as silica beads, and acidic conditions for binding sulfonated DNA to silica surfaces. La présente invention concerne des procédés et des systèmes de purification d'ADN sulfoné, spécifiquement, chimiquement modifié. De plus, l'invention concerne des procédés et des systèmes de conversion au bisulfite avec une purification améliorée d'ADN sulfoné à l'aide de supports de silice, tels que des billes de silice, et des conditions acides pour lier de l'ADN sulfoné à des surfaces de silice.