PHOSPHOLIPASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM

Phosphatidyhnositol-specific phospholipase C (PI-PLC) enzymes, nucleic acids encoding them, antibodies that bind specifically to them, and methods for making and using them are disclosed lndustnal methods and products comprising use of these phosphohpases are also provided Further provided are methods for hydration of non hydratable phospholipids (NHPs) within a lipid matrix to enable migration of NHPs to an oil-water interface, thereby allowing the NHPs to be reacted and/or removed from the lipids Further provided is a method for removing NHPs, hydratable phospholipids, and lecithins from vegetable oils to produce a degummed oil or fat product that can be used for food production and/or non-food applications.