NOVEL CLASS 2 TYPE II AND TYPE V CRISPR-CAS RNA-GUIDED ENDONUCLEASES

Provided herein are novel Class 2 Type II and Type V CRISPR-Cas RNA-guided endonucleases, e.g. Cas9 and Casl2 endonucleases, and systems comprising the same. Provided also are methods of making, and methods of use thereof. Exemplary methods of use include modifying target DNAs and detecting targetin...

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Bibliographische Detailangaben
Hauptverfasser:	GIMENEZ, CARLA ALEJANDRA, FARIAS, MARIA EUGENIA, GOYTIA, FRANCO, PEREYRA BONNET, FEDERICO ALBERTO, REPIZO, GUILLERMO DANIEL, CURTI, LUCIA ANA
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Provided herein are novel Class 2 Type II and Type V CRISPR-Cas RNA-guided endonucleases, e.g. Cas9 and Casl2 endonucleases, and systems comprising the same. Provided also are methods of making, and methods of use thereof. Exemplary methods of use include modifying target DNAs and detecting targeting DNAs, useful for therapeutic and diagnostic applications. Some of the diagnostic applications may utilise the collateral nuclease activity of an enzyme bound to a target sequence. La présente invention concerne de nouvelles endonucléases guidées par un ARN CRISPR-Cas de classe 2 de type II et de type V, par exemple des endonucléases Cas9 et Casl2, et des systèmes les comprenant. L'invention concerne également des procédés de fabrication et des procédés d'utilisation de celles-ci. Des exemples de procédés d'utilisation comprennent la modification d'ADN cibles et la détection d'ADN de ciblage, utiles pour des applications thérapeutiques et de diagnostic. Certaines des applications de diagnostic peuvent utiliser l'activité nucléase collatérale d'une enzyme liée à une séquence cible.