CELL CULTURE METHODS

A method of cell culture includes (i) culturing cells in a cell culture medium, and (ii) maintaining at least one metabolite selected from aconitic acid (AA), leucinic acid (HICA), cytidine monophosphate (CMP), methylsuccinic acid (MSA), trigonelline (TRI) and N-acetylputrescinium (NAP) below an inh...

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Bibliographische Detailangaben
Hauptverfasser: KUANG, BINGYU, GALBRAITH, SHAUN, YOON, SEONGKYU
Format: Patent
Sprache:eng ; fre
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Zusammenfassung:A method of cell culture includes (i) culturing cells in a cell culture medium, and (ii) maintaining at least one metabolite selected from aconitic acid (AA), leucinic acid (HICA), cytidine monophosphate (CMP), methylsuccinic acid (MSA), trigonelline (TRI) and N-acetylputrescinium (NAP) below an inhibitory concentration in the cell culture medium for the at least one metabolite. Procédé de culture cellulaire comprenant (i) la culture de cellules dans un milieu de culture cellulaire, et (ii) le maintien d'au moins un métabolite choisi parmi l'acide aconitique (AA), l'acide leucinique (HICA), la cytidine monophosphate (CMP), l'acide méthylsuccinique (MSA), la trigonelline (TRI) et N-acétylescinium (NAP) en dessous d'une concentration inhibitrice dans le milieu de culture cellulaire pour ledit au moins un métabolite.