NOVEL PROCESSES FOR THE PRODUCTION OF OLIGONUCLEOTIDES

Disclosed herein are novel processes for the production of oligonucleotides that are suitable for use in the production of chemically modified oligonucleotides, such as those for use in therapy, said processes comprising: a) providing a template oligonucleotide (I) complimentary to the sequence of t...

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Bibliographische Detailangaben
Hauptverfasser:	THOMAS, PAMELA JOAN, CRAMERI, ANDREAS, TEW, DAVID GRAHAM, LOVELOCK, SARAH LOUISE, HILL, MALCOLM LEITHHEAD, SCHOBER, MARKUS
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Disclosed herein are novel processes for the production of oligonucleotides that are suitable for use in the production of chemically modified oligonucleotides, such as those for use in therapy, said processes comprising: a) providing a template oligonucleotide (I) complimentary to the sequence of the product, said template having properties that allow it to be separated from the product; b) providing a pool of oligonucleotides (II); c) contacting (I) and (II) in conditions to allow annealing; d) changing the conditions to separate any impurities, comprising denaturing the annealed template and impurity oligonucleotide strands and separating the impurities; and e) changing the conditions to separate the product, comprising denaturing the annealed template and product oligonucleotide strands and separating the product. L'invention concerne de nouveaux procédés de production d'oligonucléotides pouvant être utilisés dans la production d'oligonucléotides modifiés chimiquement, tels que ceux utilisés en thérapie.