METHODS FOR PROTEIN TYROSINE PHOSPHORYLATION PROFILING WITH VARIANT SH2 DOMAINS

METHODS FOR PROTEIN TYROSINE PHOSPHORYLATION PROFILING WITH VARIANT SH2 DOMAINS

There is provided method of profiling protein tyrosine phosphorylation of a sample, the method comprising: contacting the sample with an SH2 Superbinder in order to bind pTyr-including peptides contained in the sample with the SH2 Superbinder; isolating the bound pTyr-including peptides from the sam...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: LI, SHUNNG, LIU, XUGUANG
Format: Patent
Sprache:eng ; fre
Schlagworte:
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Beschreibung
Zusammenfassung:There is provided method of profiling protein tyrosine phosphorylation of a sample, the method comprising: contacting the sample with an SH2 Superbinder in order to bind pTyr-including peptides contained in the sample with the SH2 Superbinder; isolating the bound pTyr-including peptides from the sample; and identifying the isolated pTyr-including peptides. L'invention porte sur un procédé de profilage de la phosphorylation de la protéine tyrosine d'un échantillon, le procédé comprenant : la mise en contact de l'échantillon avec un superliant SH2 afin de lier les peptides comprenant pTyr contenus dans l'échantillon avec le superliant SH2; l'isolement des peptides liés contenant pTyr à partir de l'échantillon; et l'identification des peptides isolés contenant pTyr.