PLURIPOTENT STEM CELL EXPANSION AND PASSAGE USING A STIRRED TANK BIOREACTOR

PLURIPOTENT STEM CELL EXPANSION AND PASSAGE USING A STIRRED TANK BIOREACTOR

Provided herein are novel methods for expansion and passaging of cell aggregates comprising stem cells and/or differentiated cells and comprising the use of closed systems in stirred tank bioreactors. The methods of the invention permit closed system serial passage expansion of pluripotent stem cell...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: ZHANG, XIAOHUA, CONWAY, KENNETH ROGER, DAVIS, BRIAN MICHAEL
Format: Patent
Sprache:eng ; fre
Schlagworte:
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Provided herein are novel methods for expansion and passaging of cell aggregates comprising stem cells and/or differentiated cells and comprising the use of closed systems in stirred tank bioreactors. The methods of the invention permit closed system serial passage expansion of pluripotent stem cells and/or progeny thereof with associated pluripotency markers and differentiation potential. L'invention concerne de nouvelles méthodes destinées à l'expansion et au repiquage d'agrégats cellulaires comprenant des cellules souches et/ou des cellules différenciées et comprenant l'utilisation de systèmes clos sur des bioréacteurs à plateforme basculante. Les méthodes de l'invention permettent une expansion du repiquage en série en système clos des cellules souches pluripotentes et/ou de leurs descendances avec des marqueurs pluripotents et un potentiel de différenciation.