MEDIA AND FERMENTATION METHODS FOR PRODUCING POLYSACCHARIDES IN BACTERIAL CELL CULTURE

The present invention relates to media and fermentation methods for producing polysaccharides in bacterial cell culture. In one aspect, the invention relates to a complex culture medium comprising a vegetable hydrolysate, a yeast extract, and a carbon source. In another aspect, the invention relates...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	KINROSS, JONATHAN PATRICK, PATEL-BROWN, SUJATA KAUSHIKBHAI, HANSON, MICHAEL ALLEN, SUN, WEIQIANG, TOMASELLO, PETER ANTHONY, LOMBERK, SCOTT ELLIS, LASKO, DANIEL R, DESAI, SUNIL GURURAO, LOTVIN, JASON ARNOLD
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The present invention relates to media and fermentation methods for producing polysaccharides in bacterial cell culture. In one aspect, the invention relates to a complex culture medium comprising a vegetable hydrolysate, a yeast extract, and a carbon source. In another aspect, the invention relates to a defined media having a total amino acid concentration greater than about 50 mM. A further aspect of the invention relates to the use of fed batch and perfusion fermentation methods for cultivating polysaccharide- producing bacteria. La présente invention concerne des milieux et des procédés de fermentation pour produire des polysaccharides dans une culture de cellules bactériennes. Dans un aspect, l'invention concerne un milieu de culture complexe comprenant un hydrolysat végétal, un extrait de levure et une source de carbone. Dans un autre aspect, l'invention concerne un milieu défini ayant une concentration totale en acides aminés supérieure à environ 50 mM. Un autre aspect de l'invention concerne l'utilisation de procédés de fermentation à alimentation discontinue et à perfusion pour cultiver des bactéries produisant des polysaccharides.