LABELED DEOXYNUCLEOSIDE TRIPHOSPHATES, USES THEREOF AND METHODS FOR THEIR PREPARATION

The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3'-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxyn...

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Bibliographische Detailangaben
Hauptverfasser:	OLEJNIK, JERZY, MARMA, MONG SANO, KORBOUKH, ILIA
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3'-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis. La présente invention concerne des procédés, des compositions, des mélanges et des kits utilisant des désoxynucléoside triphosphates comprenant une position 3'-O coiffée par un groupe comprenant du méthylènedisulfure comme groupe protecteur clivable et un marqueur détectable relié de manière réversible à la nucléobase dudit désoxynucléoside. Lesdits composés offrent de nouvelles possibilités pour des technologies de séquençage futures, comprenant entre autres le séquençage par synthèse.