A MICROFLUIDIC DEVICE AND METHODS FOR PROCESSING POLYNUCLEOTIDE-CONTAINING SAMPLES

Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that regain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under...

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Bibliographische Detailangaben
Hauptverfasser:	BRAHMASANDRA, SUNDARESH N, WU, BETTY, PHADKE, NIKHIL, ALTHAUS, JOHN S, HANDIQUE, KALYAN
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	ACTUATING-FLOATS APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY BLASTING CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMISTRY COCKS COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DEVICES FOR VENTING OR AERATING ENGINEERING ELEMENTS AND UNITS ENZYMOLOGY GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVEFUNCTIONING OF MACHINES OR INSTALLATIONS HEATING LIGHTING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MECHANICAL ENGINEERING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TAPS THERMAL INSULATION IN GENERAL TRANSPORTING VALVES VINEGAR WEAPONS WINE
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Beschreibung
Zusammenfassung:	Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that regain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed. Des méthodes et des systèmes servant à traiter des polynucléotides (par exemple, lADN) sont décrits. Une région de traitement comprend une ou plusieurs surfaces (par exemple, des surfaces de particules) modifiées avec des ligands qui retiennent des polynucléotides selon un premier ensemble de conditions (par exemple, la température et le pH) et libèrent les polynucléotides dans un deuxième ensemble de conditions (par exemple, une température plus élevée et/ou un pH plus basique). La région de traitement peut être utilisée, par exemple, pour concentrer des polynucléotides dun échantillon et/ou séparer des inhibiteurs de réactions damplification des polynucléotides. Des dispositifs microfluidiques possédant une région de traitement sont aussi décrits.