RAPID CONCENTRATION, RECOVERY AND DETECTION OF PATHOGENS IN FOOD SAMPLES

Methods for rapidly concentrating a food sample for efficient detection of bacteria are disclosed. A microfiltration approach followed by centrifugation was used to concentrate the cells with an enzyme (e.g., a protease) added at the beginning of the process to facilitate more efficient micro-filter...

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Bibliographische Detailangaben
Hauptverfasser:	FOSTER, KIRK SOLON, XIMINES, EDUARDO DE AQUINO, JONES, JAMES THOMAS, TUNGARE, ALISHA, LADISCH, MICHAEL RALPH, HARDENSTEIN, JAYCEY, LIU, XINGYA (LINDA), DEERING, AMANDA, KU, SEOCKMO, KREKE, THOMAS RICHARD
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Methods for rapidly concentrating a food sample for efficient detection of bacteria are disclosed. A microfiltration approach followed by centrifugation was used to concentrate the cells with an enzyme (e.g., a protease) added at the beginning of the process to facilitate more efficient micro-filtering. The enzyme was found to have no significant effect on cell viability. L'invention concerne des procédés permettant de concentrer rapidement un échantillon alimentaire pour la détection efficace de bactéries. Une approche de microfiltration, suivie par une centrifugation, est utilisée pour concentrer les cellules avec une enzyme (par exemple une protéase) ajoutée au début du processus pour que la microfiltration soit plus efficace. L'enzyme s'avère n'avoir aucun effet significatif sur la viabilité cellulaire.