METHOD OF ESTIMATING THE AMOUNT OF A METHYLATED LOCUS IN A SAMPLE
A method of estimating the amount of a methylated locus is provided. In certain embodiments the method comprises: digesting a nucleic acid sample that contains both unmethylated and methylated copies of a genomic locus with an MspJI family member to produce a population of fragments that are in the...
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| creator | BANER, JOHAN DAHL, CARL OSCAR FREDRIK ERICSSON, OLOF JOHN |
| description | A method of estimating the amount of a methylated locus is provided. In certain embodiments the method comprises: digesting a nucleic acid sample that contains both unmethylated and methylated copies of a genomic locus with an MspJI family member to produce a population of fragments that are in the range of 20-40 nucleotides in length, ligating adaptor sequence A and adaptor sequence B to the respective ends of a target fragment of sequence X, and quantifying the amount of ligation products of formula A-X-B. A kit for performing the method is also provided.
L'invention concerne un procédé d'estimation de la quantité d'un locus méthylé. Selon certains modes de réalisation, le procédé comprend : la digestion d'un échantillon d'acide nucléique contenant à la fois des copies non méthylées et méthylées d'un locus génomique avec un membre de la famille MspJI, afin de produire une population de fragments dans la plage de 20 à 40 nucléotides de longueur, la ligature d'une séquence d'adaptateur A et d'une séquence d'adaptateur B aux extrémités respectives d'un fragment cible de la séquence X, et la quantification de la quantité de produits de ligature de formule A-X-B. L'invention concerne également un kit destiné à mettre en uvre le procédé. |
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L'invention concerne un procédé d'estimation de la quantité d'un locus méthylé. Selon certains modes de réalisation, le procédé comprend : la digestion d'un échantillon d'acide nucléique contenant à la fois des copies non méthylées et méthylées d'un locus génomique avec un membre de la famille MspJI, afin de produire une population de fragments dans la plage de 20 à 40 nucléotides de longueur, la ligature d'une séquence d'adaptateur A et d'une séquence d'adaptateur B aux extrémités respectives d'un fragment cible de la séquence X, et la quantification de la quantité de produits de ligature de formule A-X-B. L'invention concerne également un kit destiné à mettre en uvre le procédé.</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230307&DB=EPODOC&CC=CA&NR=2956883C$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230307&DB=EPODOC&CC=CA&NR=2956883C$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>BANER, JOHAN</creatorcontrib><creatorcontrib>DAHL, CARL OSCAR FREDRIK</creatorcontrib><creatorcontrib>ERICSSON, OLOF JOHN</creatorcontrib><title>METHOD OF ESTIMATING THE AMOUNT OF A METHYLATED LOCUS IN A SAMPLE</title><description>A method of estimating the amount of a methylated locus is provided. In certain embodiments the method comprises: digesting a nucleic acid sample that contains both unmethylated and methylated copies of a genomic locus with an MspJI family member to produce a population of fragments that are in the range of 20-40 nucleotides in length, ligating adaptor sequence A and adaptor sequence B to the respective ends of a target fragment of sequence X, and quantifying the amount of ligation products of formula A-X-B. A kit for performing the method is also provided.
L'invention concerne un procédé d'estimation de la quantité d'un locus méthylé. Selon certains modes de réalisation, le procédé comprend : la digestion d'un échantillon d'acide nucléique contenant à la fois des copies non méthylées et méthylées d'un locus génomique avec un membre de la famille MspJI, afin de produire une population de fragments dans la plage de 20 à 40 nucléotides de longueur, la ligature d'une séquence d'adaptateur A et d'une séquence d'adaptateur B aux extrémités respectives d'un fragment cible de la séquence X, et la quantification de la quantité de produits de ligature de formule A-X-B. L'invention concerne également un kit destiné à mettre en uvre le procédé.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZHD0dQ3x8HdR8HdTcA0O8fR1DPH0c1cI8XBVcPT1D_ULAUk4KoAURfo4hri6KPj4O4cGK3j6AUWDHX0DfFx5GFjTEnOKU3mhNDeDvJtriLOHbmpBfnxqcUFicmpeakm8s6ORpamZhYWxszFhFQD9Mimn</recordid><startdate>20230307</startdate><enddate>20230307</enddate><creator>BANER, JOHAN</creator><creator>DAHL, CARL OSCAR FREDRIK</creator><creator>ERICSSON, OLOF JOHN</creator><scope>EVB</scope></search><sort><creationdate>20230307</creationdate><title>METHOD OF ESTIMATING THE AMOUNT OF A METHYLATED LOCUS IN A SAMPLE</title><author>BANER, JOHAN ; DAHL, CARL OSCAR FREDRIK ; ERICSSON, OLOF JOHN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CA2956883C3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>BANER, JOHAN</creatorcontrib><creatorcontrib>DAHL, CARL OSCAR FREDRIK</creatorcontrib><creatorcontrib>ERICSSON, OLOF JOHN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>BANER, JOHAN</au><au>DAHL, CARL OSCAR FREDRIK</au><au>ERICSSON, OLOF JOHN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHOD OF ESTIMATING THE AMOUNT OF A METHYLATED LOCUS IN A SAMPLE</title><date>2023-03-07</date><risdate>2023</risdate><abstract>A method of estimating the amount of a methylated locus is provided. In certain embodiments the method comprises: digesting a nucleic acid sample that contains both unmethylated and methylated copies of a genomic locus with an MspJI family member to produce a population of fragments that are in the range of 20-40 nucleotides in length, ligating adaptor sequence A and adaptor sequence B to the respective ends of a target fragment of sequence X, and quantifying the amount of ligation products of formula A-X-B. A kit for performing the method is also provided.
L'invention concerne un procédé d'estimation de la quantité d'un locus méthylé. Selon certains modes de réalisation, le procédé comprend : la digestion d'un échantillon d'acide nucléique contenant à la fois des copies non méthylées et méthylées d'un locus génomique avec un membre de la famille MspJI, afin de produire une population de fragments dans la plage de 20 à 40 nucléotides de longueur, la ligature d'une séquence d'adaptateur A et d'une séquence d'adaptateur B aux extrémités respectives d'un fragment cible de la séquence X, et la quantification de la quantité de produits de ligature de formule A-X-B. L'invention concerne également un kit destiné à mettre en uvre le procédé.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | METHOD OF ESTIMATING THE AMOUNT OF A METHYLATED LOCUS IN A SAMPLE |
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