XYLANASE VARIANTS HAVING INCREASED THERMOSTABILITY
The present inventions relates to a modified GH10 xylanase enzyme or a fragment thereof having xylanase activity wherein said modified GH10 xylanase or fragment thereof has increased thermostability compared with a parent GH10 xylanase enzyme, the parent GH10 xylanase having been modified at, at lea...
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| creator | HENDRIK A VAN TUIJL, JAN KOOPS, BART LORENTSEN, RIKKE HOEEGH ARENT LUND, SUSAN NIKOLAEV, IGOR |
| description | The present inventions relates to a modified GH10 xylanase enzyme or a fragment thereof having xylanase activity wherein said modified GH10 xylanase or fragment thereof has increased thermostability compared with a parent GH10 xylanase enzyme, the parent GH10 xylanase having been modified at, at least, two of the following positions 7, 33, 79, 217 and 298, wherein the numbering is based on the amino acid numbering of FveXyn4 (SEQ ID No. 1). In particular, the modified xylanase enzyme according to the present invention has the following modifications: N7D; T33V; K79Y, V, F, I, L or M (preferably K79Y, F or V, more preferably Y); A217Q, E, P, D or M (preferably A217Q, E or P, more preferably Q); and T298Y, F or W (preferably Y or F, more preferably Y).
La présente invention concerne une enzyme xylanase GH10 modifiée ou un fragment de celle-ci présentant une activité de xylanase, ladite xylanase GH10 modifiée ou un fragment de celle-ci possédant une thermostabilité accrue comparativement à une enzyme xylanase GH10 mère, la xylanase GH10 mère ayant été modifiée au niveau d'au moins deux des positions suivantes 7, 33, 79, 217 et 298, la numérotation étant basée sur la numérotation des acides aminés de FveXyn4 (SEQ ID No1). En particulier, l'enzyme xylanase modifiée selon la présente invention présente les modifications suivantes : N7D ; T33V ; K79Y, V, F, I, L ou M (de préférence K79Y, F ou V, plus préférentiellement Y) ; A217Q, E, P, D ou M (de préférence A217Q, E ou P, plus préférentiellement Q) ; et T298Y, F ou W (de préférence Y ou F, plus préférentiellement Y). |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CA2937043C</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CA2937043C</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CA2937043C3</originalsourceid><addsrcrecordid>eNrjZDCKiPRx9HMMdlUIcwzydPQLCVbwcAzz9HNX8PRzDnIFSrgohHi4Bvn6B4c4Onn6eIZE8jCwpiXmFKfyQmluBnk31xBnD93Ugvz41OKCxOTUvNSSeGdHI0tjcwMTY2djwioAC2MmUw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>XYLANASE VARIANTS HAVING INCREASED THERMOSTABILITY</title><source>esp@cenet</source><creator>HENDRIK A VAN TUIJL, JAN ; KOOPS, BART ; LORENTSEN, RIKKE HOEEGH ; ARENT LUND, SUSAN ; NIKOLAEV, IGOR</creator><creatorcontrib>HENDRIK A VAN TUIJL, JAN ; KOOPS, BART ; LORENTSEN, RIKKE HOEEGH ; ARENT LUND, SUSAN ; NIKOLAEV, IGOR</creatorcontrib><description>The present inventions relates to a modified GH10 xylanase enzyme or a fragment thereof having xylanase activity wherein said modified GH10 xylanase or fragment thereof has increased thermostability compared with a parent GH10 xylanase enzyme, the parent GH10 xylanase having been modified at, at least, two of the following positions 7, 33, 79, 217 and 298, wherein the numbering is based on the amino acid numbering of FveXyn4 (SEQ ID No. 1). In particular, the modified xylanase enzyme according to the present invention has the following modifications: N7D; T33V; K79Y, V, F, I, L or M (preferably K79Y, F or V, more preferably Y); A217Q, E, P, D or M (preferably A217Q, E or P, more preferably Q); and T298Y, F or W (preferably Y or F, more preferably Y).
La présente invention concerne une enzyme xylanase GH10 modifiée ou un fragment de celle-ci présentant une activité de xylanase, ladite xylanase GH10 modifiée ou un fragment de celle-ci possédant une thermostabilité accrue comparativement à une enzyme xylanase GH10 mère, la xylanase GH10 mère ayant été modifiée au niveau d'au moins deux des positions suivantes 7, 33, 79, 217 et 298, la numérotation étant basée sur la numérotation des acides aminés de FveXyn4 (SEQ ID No1). En particulier, l'enzyme xylanase modifiée selon la présente invention présente les modifications suivantes : N7D ; T33V ; K79Y, V, F, I, L ou M (de préférence K79Y, F ou V, plus préférentiellement Y) ; A217Q, E, P, D ou M (de préférence A217Q, E ou P, plus préférentiellement Q) ; et T298Y, F ou W (de préférence Y ou F, plus préférentiellement Y).</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; BREWING OF BEER ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; FODDER ; FOODS OR FOODSTUFFS ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS ; SPIRITS ; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231024&DB=EPODOC&CC=CA&NR=2937043C$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20231024&DB=EPODOC&CC=CA&NR=2937043C$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HENDRIK A VAN TUIJL, JAN</creatorcontrib><creatorcontrib>KOOPS, BART</creatorcontrib><creatorcontrib>LORENTSEN, RIKKE HOEEGH</creatorcontrib><creatorcontrib>ARENT LUND, SUSAN</creatorcontrib><creatorcontrib>NIKOLAEV, IGOR</creatorcontrib><title>XYLANASE VARIANTS HAVING INCREASED THERMOSTABILITY</title><description>The present inventions relates to a modified GH10 xylanase enzyme or a fragment thereof having xylanase activity wherein said modified GH10 xylanase or fragment thereof has increased thermostability compared with a parent GH10 xylanase enzyme, the parent GH10 xylanase having been modified at, at least, two of the following positions 7, 33, 79, 217 and 298, wherein the numbering is based on the amino acid numbering of FveXyn4 (SEQ ID No. 1). In particular, the modified xylanase enzyme according to the present invention has the following modifications: N7D; T33V; K79Y, V, F, I, L or M (preferably K79Y, F or V, more preferably Y); A217Q, E, P, D or M (preferably A217Q, E or P, more preferably Q); and T298Y, F or W (preferably Y or F, more preferably Y).
La présente invention concerne une enzyme xylanase GH10 modifiée ou un fragment de celle-ci présentant une activité de xylanase, ladite xylanase GH10 modifiée ou un fragment de celle-ci possédant une thermostabilité accrue comparativement à une enzyme xylanase GH10 mère, la xylanase GH10 mère ayant été modifiée au niveau d'au moins deux des positions suivantes 7, 33, 79, 217 et 298, la numérotation étant basée sur la numérotation des acides aminés de FveXyn4 (SEQ ID No1). En particulier, l'enzyme xylanase modifiée selon la présente invention présente les modifications suivantes : N7D ; T33V ; K79Y, V, F, I, L ou M (de préférence K79Y, F ou V, plus préférentiellement Y) ; A217Q, E, P, D ou M (de préférence A217Q, E ou P, plus préférentiellement Q) ; et T298Y, F ou W (de préférence Y ou F, plus préférentiellement Y).</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>BREWING OF BEER</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>FODDER</subject><subject>FOODS OR FOODSTUFFS</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</subject><subject>SPIRITS</subject><subject>THEIR TREATMENT, NOT COVERED BY OTHER CLASSES</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDCKiPRx9HMMdlUIcwzydPQLCVbwcAzz9HNX8PRzDnIFSrgohHi4Bvn6B4c4Onn6eIZE8jCwpiXmFKfyQmluBnk31xBnD93Ugvz41OKCxOTUvNSSeGdHI0tjcwMTY2djwioAC2MmUw</recordid><startdate>20231024</startdate><enddate>20231024</enddate><creator>HENDRIK A VAN TUIJL, JAN</creator><creator>KOOPS, BART</creator><creator>LORENTSEN, RIKKE HOEEGH</creator><creator>ARENT LUND, SUSAN</creator><creator>NIKOLAEV, IGOR</creator><scope>EVB</scope></search><sort><creationdate>20231024</creationdate><title>XYLANASE VARIANTS HAVING INCREASED THERMOSTABILITY</title><author>HENDRIK A VAN TUIJL, JAN ; KOOPS, BART ; LORENTSEN, RIKKE HOEEGH ; ARENT LUND, SUSAN ; NIKOLAEV, IGOR</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CA2937043C3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>BREWING OF BEER</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>FODDER</topic><topic>FOODS OR FOODSTUFFS</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</topic><topic>SPIRITS</topic><topic>THEIR TREATMENT, NOT COVERED BY OTHER CLASSES</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>HENDRIK A VAN TUIJL, JAN</creatorcontrib><creatorcontrib>KOOPS, BART</creatorcontrib><creatorcontrib>LORENTSEN, RIKKE HOEEGH</creatorcontrib><creatorcontrib>ARENT LUND, SUSAN</creatorcontrib><creatorcontrib>NIKOLAEV, IGOR</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HENDRIK A VAN TUIJL, JAN</au><au>KOOPS, BART</au><au>LORENTSEN, RIKKE HOEEGH</au><au>ARENT LUND, SUSAN</au><au>NIKOLAEV, IGOR</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>XYLANASE VARIANTS HAVING INCREASED THERMOSTABILITY</title><date>2023-10-24</date><risdate>2023</risdate><abstract>The present inventions relates to a modified GH10 xylanase enzyme or a fragment thereof having xylanase activity wherein said modified GH10 xylanase or fragment thereof has increased thermostability compared with a parent GH10 xylanase enzyme, the parent GH10 xylanase having been modified at, at least, two of the following positions 7, 33, 79, 217 and 298, wherein the numbering is based on the amino acid numbering of FveXyn4 (SEQ ID No. 1). In particular, the modified xylanase enzyme according to the present invention has the following modifications: N7D; T33V; K79Y, V, F, I, L or M (preferably K79Y, F or V, more preferably Y); A217Q, E, P, D or M (preferably A217Q, E or P, more preferably Q); and T298Y, F or W (preferably Y or F, more preferably Y).
La présente invention concerne une enzyme xylanase GH10 modifiée ou un fragment de celle-ci présentant une activité de xylanase, ladite xylanase GH10 modifiée ou un fragment de celle-ci possédant une thermostabilité accrue comparativement à une enzyme xylanase GH10 mère, la xylanase GH10 mère ayant été modifiée au niveau d'au moins deux des positions suivantes 7, 33, 79, 217 et 298, la numérotation étant basée sur la numérotation des acides aminés de FveXyn4 (SEQ ID No1). En particulier, l'enzyme xylanase modifiée selon la présente invention présente les modifications suivantes : N7D ; T33V ; K79Y, V, F, I, L ou M (de préférence K79Y, F ou V, plus préférentiellement Y) ; A217Q, E, P, D ou M (de préférence A217Q, E ou P, plus préférentiellement Q) ; et T298Y, F ou W (de préférence Y ou F, plus préférentiellement Y).</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY BREWING OF BEER CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FODDER FOODS OR FOODSTUFFS HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE |
| title | XYLANASE VARIANTS HAVING INCREASED THERMOSTABILITY |
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