COMPOSITIONS AND METHODS FOR DETECTING AND QUANTIFYING HOST CELL PROTEIN IN CELL LINES AND RECOMBINANT POLYPEPTIDE PRODUCTS

Monoclonal and polyclonal antibodies that bind hamster phospholipase B-like 2 are provided. Also provided are methods for detecting and quantifying hamster phospholipase B-like 2, for example, in recombinant polypeptide preparations, as well as kits for carrying out such methods. Methods of screenin...

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Hauptverfasser:	ZHU-SHIMONI, JUDITH, VANDERLAAN, MARTIN, VIJ, RAJESH, YUK, INN H, HSIAO, YIUN, LIN, MARGARET S, KRAWITZ, DENISE C, GUNAWAN, FENY
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Monoclonal and polyclonal antibodies that bind hamster phospholipase B-like 2 are provided. Also provided are methods for detecting and quantifying hamster phospholipase B-like 2, for example, in recombinant polypeptide preparations, as well as kits for carrying out such methods. Methods of screening or selecting host cell lines or recombinant polypeptide-expressing cell lines that express low levels of hamster phospholipase B-like 2 are also provided. L'invention concerne des anticorps monoclonaux et polyclonaux qui se lient à la phospholipase B-like 2 du hamster. L'invention concerne également des méthodes permettant de détecter et de quantifier la phospholipase B-like 2 du hamster, par exemple dans des préparations de polypeptides recombinés, ainsi que des kits permettant de mettre en uvre lesdites méthodes. L'invention concerne par ailleurs des méthodes de criblage ou de sélection de lignées cellulaires hôtes ou de lignées cellulaires exprimant les polypeptides recombinés qui expriment des taux faibles de phospholipase B-like 2 du hamster.