DOWN-REGULATION OF GENE EXPRESSION USING ARTIFICIAL MICRORNAS

The field of the present disclosure relates to plant molecular biology and, in particular, to constructs and methods to down-regulate the expression of target genes. Described herein are isolated nucleic acid fragments encoding, artificial miRNAs and their use in down-regulating gene expression. In...

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Bibliographische Detailangaben
1. Verfasser:	MCGONIGLE, BRIAN
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The field of the present disclosure relates to plant molecular biology and, in particular, to constructs and methods to down-regulate the expression of target genes. Described herein are isolated nucleic acid fragments encoding, artificial miRNAs and their use in down-regulating gene expression. In one aspect of the present disclosure, an artificial miRNA precursor is achieved by modifying the deoxyribonucleotide sequence that encodes a soybean miRNA precursor. In this aspect, the miRNA and star sequences of the miRNA precursor are replaced with variable nucleotide subsequences designed to down-regulate the expression of a target gene. In another aspect of the present disclosure, a plant cell is transformed with a nucleic acid construct comprising a nucleic acid fragment that encodes an artificial miRNA precursor for reducing the expression of a target gene.