METHODS FOR ISOLATING MICROVESICLES

Current research is directed at utilizing microvesicles and the genetic and proteomic information contained therein to gain further insight into the status of cells, for example, disease state or predisposition for a disease. Accordingly, there is a need for methods of isolating microvesicles from b...

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Bibliographische Detailangaben
Hauptverfasser:	ENDERLE, DANIEL, RAMACHANDRAN, APARNA, YAN, HAOHENG, SKOG, JOHAN KARL OLOV, BERGHOFF, EMILY, WEI, TAI-FEN, NOERHOLM, MIKKEL
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Current research is directed at utilizing microvesicles and the genetic and proteomic information contained therein to gain further insight into the status of cells, for example, disease state or predisposition for a disease. Accordingly, there is a need for methods of isolating microvesicles from biological samples and methods of extracting high quality nucleic acids for accurate diagnosis of medical conditions and diseases. The present invention provides methods for isolating microvesicles comprising capturing the microvesicles on a positively charged surface functionalized with quaternary ammonium and subsequently lysing the microvesicles to extract the nucleic acids contained therein. L'invention concerne des procédés inédits d'isolement de microvésicules contenues dans un échantillon biologique, ainsi que d'extraction d'acides nucléiques contenus dans lesdites microvésicules.