NOVEL METHANOL DEHYDROGENASE ENZYMES FROM BACILLUS

The present invention relates to a nucleic acid molecule, which encodes a polypeptide having alcohol dehydrogenase activity, in particular methanol dehydrogenase activity, comprising having a nucleotide sequence selected from the group consisting of: (i) a nucleotide sequence as set forth in any one of SEQ ID NOs: 1 (mdh2-MGA3), 3 (mdh3-MGA3), or 5 (mdh2-PB1); (ii) a nucleotide sequence having at least 90% sequence identity, more particularly at least 91, 92, 93, 94, 95, 96, 97, 98 or 99% sequence identity, with a nucleotide sequence as set forth in any one of SEQ ID NOs: 1, 3 or 5; (iii) a nucleotide sequence which is degenerate with any one of the nucleotide sequences of SEQ ID NOs: 1, 3 or 5; (iv) a nucleotide sequence which is a part of the nucleotide sequence of any one of SEQ ID NOs: 1, 3 or 5, or of a nucleotide sequence which is degenerate with a sequence of SEQ ID NOs:1, 3 or 5; (v) a nucleotide sequence encoding all or part of a polypeptide whose amino acid sequence is set forth in any one of SEQ ID NOs : 2 (Mdh2-MGA3), 4 (Mdh3-MGA3) or 6 (Mdh2-PB1); and (vi) a nucleotide sequence encoding all or part of a polypeptide which has an amino acid sequence having at least 90% sequence identity, preferably at least 91, 92, 93, 94, 95, 96, 97, 98 or 99% sequence identity, with an amino acid sequence as set forth in any one of SEQ ID NOs: 2, 4 or 6; or a nucleic acid molecule comprising a nucleotide sequence which is complementary to the nucleotide sequence of any one of (i) to (vi). Also provided are recombinant constructs, vectors and host cells comprising such a nucleic acid molecule and polypeptides encoded thereby. Such molecules may advantageously be used in the genetic modification of host cells, for example to introduce or modify methanol dehydrogenase activity. La présente invention concerne une molécule d'acide nucléique, qui code pour un polypeptide présentant une activité d'alcool déshydrogénase, en particulier une activité de méthanol déshydrogénase, comprenant une séquence nucléotidique sélectionnée dans le groupe constitué par : (i) une séquence nucléotidique telle décrite dans l'une quelconque des séquences SEQ ID NO : 1 (mdh2-MGA3), 3 (mdh3-MGA3) ou 5 (mdh2-PB1) ; (ii) une séquence nucléotidique présentant au moins 90 % d'identité de séquence, plus particulièrement au moins 91, 92, 93, 94, 95, 96, 97, 98 ou 99 % d'identité de séquence, par rapport à une séquence nucléotidique telle décrite dans l'une quelconque des séquences SEQ ID NO : 1