USE OF A RARE-CLEAVING DOUBLE STRAND BREAK INDUCING ENZYME FOR TARGETED DNA INSERTION IN PLANTS

Methods and means are provided to improve targeted DNA insertion in plants using rare-cleaving "double stranded break" inducing enzymes. Also provided are improved I-SceI encoding nucleotide sequences. Des méthodes et des moyens sont proposés pour améliorer linsertion dADN ciblée dans des...

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Hauptverfasser:	VANDERSTRAETEN, CHANTAL, RUITER, RENE, D'HALLUIN, KATHLEEN
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Zusammenfassung:	Methods and means are provided to improve targeted DNA insertion in plants using rare-cleaving "double stranded break" inducing enzymes. Also provided are improved I-SceI encoding nucleotide sequences. Des méthodes et des moyens sont proposés pour améliorer linsertion dADN ciblée dans des plantes en utilisant des enzymes de clivage rare induisant un bris de double brin. Des séquences de nucléotides codantes I-SceI améliorées sont proposées.