OXIDATION AND AMINATION OF SECONDARY ALCOHOLS

The invention relates to a method comprising the following steps: a) providing a secondary alcohol; b) oxidizing the secondary alcohol by bringing said alcohol into contact with an NAD(P)+-dependent alcohol dehydrogenase; and c) bringing the oxidation product of step a) into contact with a transamin...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	SATTLER, JOHANN H, KROUTIL, WOLFGANG, POTTER, MARKUS, PFEFFER, JAN CHRISTOPH, LERCHNER, ALEXANDRA, TAUBER, KATHARINA CHRISTIN, HAAS, THOMAS, SKERRA, ARNE, SCHAFFER, STEFFEN
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a method comprising the following steps: a) providing a secondary alcohol; b) oxidizing the secondary alcohol by bringing said alcohol into contact with an NAD(P)+-dependent alcohol dehydrogenase; and c) bringing the oxidation product of step a) into contact with a transaminase, wherein the NAD(P)+-dependent alcohol dehydrogenase and/or the transaminase are/is a recombinant or isolated enzyme. The invention further relates to a whole cell catalyst for carrying out the method and to the use of such a whole cell catalyst for oxidizing a secondary alcohol.