SYSTEMS AND METHODS TO PERFORM ASSAYS FOR DETECTING OR QUANTIFYING ANALYTES WITHIN SAMPLES

A method for reducing a nucleic acid amplification inhibitor in a receptacle comprises the steps of coating a portion of an inner surface of a receptacle with a surface treating agent, such as an oil, that reduces the amount of an amplification inhibitor adhering to the inner surface during a proces...

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Bibliographische Detailangaben
Hauptverfasser:	MACIOSZEK, JERZY A, LAIR, GARY D, KNIGHT, BYRON J, HEINZ, ROBERT E, LI, FLORENCE F, SCALESE, ROBERT F, NGUYEN, THANH N, CARRICK, JAMES M, LI, HAITAO, DAVIS, CHRISTOPHER BRANDON, LIGHT, JAMES P
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method for reducing a nucleic acid amplification inhibitor in a receptacle comprises the steps of coating a portion of an inner surface of a receptacle with a surface treating agent, such as an oil, that reduces the amount of an amplification inhibitor adhering to the inner surface during a process of isolating a target nucleic acid within the receptacle, washing the target nucleic acid, and amplifying a target sequence of the target nucleic acid. The surface treating agent does not inhibit amplification of the target sequence. L'invention a trait à une méthode pour réduire un inhibiteur d'amplification d'acide nucléique dans un réceptacle qui comprend les étapes de recouvrement d'une portion d'une surface interne d'un réceptacle avec un agent de traitement de surface, tel qu'une huile, qui réduit l'adhérence d'un inhibiteur d'amplification à la surface interne pendant un processus d'isolation d'un acide nucléique cible dans le réceptacle, de lavage de l'acide nucléique cible, et d'amplification d'une séquence cible de l'acide nucléique cible. L'agent de traitement de surface n'inhibe pas l'amplification de la séquence cible.