PROCESS FOR THE IMPROVED SEPARATION OF A HYDROPHOBIC ORGANIC SOLUTION FROM AN AQUEOUS CULTURE MEDIUM

The present application relates to a process for the improved separation of a hydrophobic organic solution from an aqueous culture medium comprising the steps, provision of an aqueous culture medium comprising a metabolically active cell, contacting of the aqueous culture medium with a hydrophobic o...

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Bibliographische Detailangaben
Hauptverfasser:	ERHARDT, FRANK, GIELEN, JASMIN, HENNEMANN, HANS-GEORG, BERSE, KATHARINA, POETTER, MARKUS, SCHIEBELHUT, MARION, HAAS, THOMAS, WESSEL, MIRJA, SCHAFFER, STEFFEN, HAFKEMEYER, SABINE
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES BEER BIOCHEMISTRY CANDLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DETERGENTS ENZYMOLOGY ESSENTIAL OILS FATTY ACIDS THEREFROM FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFUMES PRODUCING (PRESSING, EXTRACTION), REFINING AND PRESERVINGFATS, FATTY SUBSTANCES (e.g. LANOLIN), FATTY OILS AND WAXES,INCLUDING EXTRACTION FROM WASTE MATERIALS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present application relates to a process for the improved separation of a hydrophobic organic solution from an aqueous culture medium comprising the steps, provision of an aqueous culture medium comprising a metabolically active cell, contacting of the aqueous culture medium with a hydrophobic organic solution, separation of the hydrophobic organic solution from the aqueous culture medium, the cell having a decreased activity, compared to its wild-type, at least of one enzyme that catalyses one of the reactions of the .beta.-oxidation of fatty acids. The invention furthermore relates to the use of a metabolically active cell that has a decreased activity, compared to its wild-type, of an enzyme that catalyses one of the reactions of the .beta.-oxidation of fatty acids, preferably of an enzyme selected from the group comprising FadA, FadB, FadD, FadL and FadE as well as variants thereof, more preferably FadE or a variant thereof, for the improved separation of a hydrophobic organic solution from an aqueous culture medium comprising the metabolically active cell. La présente invention concerne un procédé de séparation améliorée dune solution organique hydrophobe dun milieu de culture aqueux, le procédé comprenant les étapes suivantes : préparation dun milieu de culture aqueux comprenant une cellule métaboliquement active, mise en contact du milieu de culture aqueux avec une solution organique hydrophobe, et séparation de la solution organique hydrophobe du milieu de culture aqueux, la cellule présentant une activité, réduite par rapport à son type sauvage, dau moins un enzyme qui catalyse une des réactions de ß oxydation dacides gras. Linvention concerne également lutilisation dune cellule métaboliquement active qui présente une activité, réduite par rapport à son type sauvage, dun enzyme qui catalyse une des réactions de ß oxydation dacides gras, de préférence dun enzyme sélectionné dans le groupe consistant en FadA, FadB, FadD, FadL et FadE ainsi que des variantes de ceux-ci, de préférence FadE ou une variante de celui-ci, pour la séparation améliorée dune solution organique hydrophobe dun milieu de culture aqueux comprenant la cellule métaboliquement active.