ENHANCED PROTEIN PURIFICATION THROUGH A MODIFIED PROTEIN A ELUTION

ENHANCED PROTEIN PURIFICATION THROUGH A MODIFIED PROTEIN A ELUTION

The present invention provides methods for purifying a polypeptide comprising a CH2/CH3 region, comprising binding the polypeptide to Protein A and eluting with a pH gradient starting at a low pH. La présente invention porte sur des procédés de purification d'un polypeptide comprenant une régio...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: DOWD, CHRISTOPHER J, BROWN, ARICK, RADHAMOHAN, ASHA NANDINI
Format: Patent
Sprache:eng ; fre
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The present invention provides methods for purifying a polypeptide comprising a CH2/CH3 region, comprising binding the polypeptide to Protein A and eluting with a pH gradient starting at a low pH. La présente invention porte sur des procédés de purification d'un polypeptide comprenant une région CH2/CH3, comprenant la liaison du polypeptide à la protéine A et l'élution par un gradient de pH commençant à un faible pH.