ENZYME COMPLEX FROM TRICHODERMA REESEI AND P. FUNICULOSUM ENZYMES

The invention relates to an enzyme composition having beta-1,4-endoglucan hydrolase activity. The enzyme composition can be used in a process for production of a brewing mash, in the production of a malt beverage, beer and whiskey. The composition comprises a fermentation product of Trichoderma rees...

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Bibliographische Detailangaben
Hauptverfasser:	MILLER, LONE BROEND, FISH, NEVILLE MARSHALL
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	AGEING BEER BIOCHEMISTRY BREWING OF BEER CHEMISTRY CLARIFICATION COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PASTEURISATION PRESERVATION PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS PURIFICATION SPIRITS STERILISATION THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to an enzyme composition having beta-1,4-endoglucan hydrolase activity. The enzyme composition can be used in a process for production of a brewing mash, in the production of a malt beverage, beer and whiskey. The composition comprises a fermentation product of Trichoderma reesei and a fermentation product of Penicillium funiculosum comprising one or more enzymes selected from a xylanase (EC 3.2.1.8), a cellulase (EC 3.2.1.4), and a beta-glucanase (EC 3.2.1.6). At least 61 % of the beta-1,4-endoglucan hydrolase activity of the enzyme composition is from the fermentation product of Trichoderma reesei, and at most 39% of the beta-1,4-endoglucan hydrolase activity is from the fermentation product of Penicillium funiculosum, as measured by the 3,5-dinitrosalicylic acid (DNS) cellulase activity method at pH 5.0 and 50°C. The invention also relates to a process for producing the enzyme composition. L'invention porte sur un complexe enzymatique amélioré ayant une pluralité d'activités enzymatiques d'un produit d'expression obtenu par fermentation du genre Trichoderma en association avec une ou plusieurs enzymes d'une souche différente de champignon.