AN IMPROVED PROCESS FOR PEGYLATION OF PROTEINS

AN IMPROVED PROCESS FOR PEGYLATION OF PROTEINS

The present invention relates to a process for improving pegylation reaction yield of r-metHuG-CSF comprising conjugating r-metHuG-CSF to a PEG aldehyde at a free amine moiety at the N terminal end on the G-CSF in presence of a reducing agent in a pegylation buffer solution comprising a polyol havin...

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Hauptverfasser: SHEKHAWAT, RAKESH, MALPURE, PANKAJ PRABHAKAR, PATIL, NILESH DAGDU, SAKSENA, DIVYA LAL, ZAWAR, SAGAR SATYANARAYAN, KHARE, ARUNA, RANA, DILIP KUMAR, CHANDRAKESAN, MURALIDHARAN, PALIWAL, DINESH KUMAR, PAWAR, DIGAMBER SHRIPATI, MOHE, NIKHIL UMESH, TARUR, RADHARKRISHNAN VENKATSUBRAMANIAN
Format: Patent
Sprache:eng ; fre
Schlagworte:
CHEMISTRY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
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Beschreibung
Zusammenfassung:The present invention relates to a process for improving pegylation reaction yield of r-metHuG-CSF comprising conjugating r-metHuG-CSF to a PEG aldehyde at a free amine moiety at the N terminal end on the G-CSF in presence of a reducing agent in a pegylation buffer solution comprising a polyol having the formula C n H2n+2O n where n is from 3 to 6, or a carbohydrate, or a derivative thereof wherein the concentration of said polyol or carbohydrate or derivative thereof is in the range of 0.1% to 10% w/w.