CELL LYSIS REAGENT FOR ISOLATION OF RNA

RNA is extracted from cellular material with a reagent that includes heparin, a reducing agent to reduce disulfide bonds, a chelating agent, a buffer, and an alkali metal halide. The reagent does not require the use of organic solvents, and the reagent allows extraction to be performed in a relative...

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Bibliographische Detailangaben
Hauptverfasser: UGOZZOLI, LUIS, DIGES, CAMILLE M
Format: Patent
Sprache:eng ; fre
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Zusammenfassung:RNA is extracted from cellular material with a reagent that includes heparin, a reducing agent to reduce disulfide bonds, a chelating agent, a buffer, and an alkali metal halide. The reagent does not require the use of organic solvents, and the reagent allows extraction to be performed in a relatively short period of time in comparison to the prior art.