PATIENT-SPECIFIC STEM CELL LINES DERIVED FROM HUMAN PARTHENOGENETIC BLASTOCYSTS

Methods are disclosed for generating HLA homozygous parthenogenetic human stem cell (hpSC-Hhom) lines from both HLA homozygous and HLA heterozygous d onors. These hpSC-Hhom lines demonstrate typical human embryonic stem cell m orphology, expressing appropriate stem cell markers and possessing high l...

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Hauptverfasser:	REVAZOVA, ELENA S, TUROVETS, NIKOLAY A, JANUS, JEFFREY D, KUZMICHEV, LEONID N
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Methods are disclosed for generating HLA homozygous parthenogenetic human stem cell (hpSC-Hhom) lines from both HLA homozygous and HLA heterozygous d onors. These hpSC-Hhom lines demonstrate typical human embryonic stem cell m orphology, expressing appropriate stem cell markers and possessing high leve ls of alkaline phosphatase and telomerase activity. Additionally, injection of these cell lines into immunodeficient animals leads to teratoma formation . Furthermore, in the case of HLA heterozygous donors, the hpSC-Hhom lines i nherit the haplotype from only one of the donor's parents. SNP data analysis suggests that hpSC-Hhom lines derived from HLA heterozygous oocyte donors a re homozygous throughout the genome as assessed by single-nucleotide polymor phism (SNP) analysis. The protocol as disclosed minimizes the use of animal- derived components, which makes the stem cells more practical for clinical a pplication.